Pathogenic windows and rescue thresholds
Define developmental windows and minimum repair thresholds for ASD risk mutations using isogenic iPSC-derived brain organoids and assembloids.
Using isogenic iPSC-derived brain organoids and assembloids, we define pathogenic windows and minimum rescue thresholds for representative ASD risk mutations, integrating time-series single-cell multi-omics and RNA base editing.
Research aims
- Generate isogenic iPSC lines carrying representative ASD risk mutations.
- Build time-series single-cell transcriptomic and epigenomic atlases of brain organoids and assembloids.
- Map developmental stages at which mutations exert causal effects.
- Test RNA base-editing rescue at different developmental windows.
This project establishes a parameter framework to support rational intervention design for neurodevelopmental disorders.